李光鹏的主要学术成就

1.世界上首例克隆骡子--2003年3匹克隆骡问世，成果发表于?科学?杂志。2.2000年获得世界上首例初级卵母细胞核移植后的哺乳动物个体--兔。3.2001年获得世界上首例次级卵母细胞纺锤体交换后的哺乳动物个体--小鼠。4.1998年获得世界上第二例胚胎细胞克隆猪。5.数百头克隆牛的创造者。6.异种克隆大熊猫的主要参入者.1999年度国家十大科技新闻之一。7.共发表论文70多篇,其中SCI论文28篇；主编及参编学术著作4部;参与翻译专著一部。研究方向：动物克隆；核质关系；减数分裂调控;干细胞研究；转基因动物。发表论著与论文：自1990年至今，共在国内外学术期刊上发表论文70多篇,其中SCI论文28篇；主编及参编学术著作4部;参与翻译专著一部。著作：1.主编：“发育生物学原理与胚胎工程”黑龙江科学技术出版社1995；2.副主编：“受精生物学----受精机制与生殖工程”。（陈大元主编；孙青原，李光鹏副主编）。科学出版社2001；3.参编：“哺乳动物胚胎学”（秦鹏春主编）。科学出版社2001；4.WhiteKL,WoodsGL,VanderwallDK,LiGP,BunchTD.CloningtheEquine.In:EpigeneticRisksofCloning.EditedbyAkioInui,2006.pp59-70.CRCPress,Taylor&FrancisGroup,BocaRaton,Florida,USA；5.参译:“小鼠胚胎操纵实验指南”.(孙青原,陈大元主译).化学工业出版社.2006。近几年主要研究论文:1.YingLiu,Guang-PengLi,BenjaminR.Sessions,LeeF.Rickords,KennethL.WhiteandThomasD.Bunch.NicotineInducesMultinuclearFormationandCausesAberrantEmbryonicDevelopmentinBovine.MolReprodDev.2007,inpress.2.LiuY,LiGP,WhiteKL,RickordsLF,SessionsBR,AstonKI,BunchTD.Nicotinealtersbovineoocytemeiosisandaffectssubsequentembryonicdevelopment.MolReprodDev.2007Apr17;[Epubaheadofprint],PMID:17440977[PubMed-assuppliedbypublisher].3.PateBJ,WhiteKL,WingerQA,RickordsLF,AstonKI,SessonsBR,LiGP,CampbellKD,WeimerB,BunchTD.2007.Specificintegrinsubunitsinbovineoocytes,includingnovelsequencesforalpha6andbeta3subunits.MolReprodDev.2007May;74(5):600-7.4.LiuY,LiGP,RickordsLF,WhiteKL,SessionsBR,AstonKI,BunchTD.Effectofnicotineoninvitromaturationofbovineoocytes.AnimReprodSci.2006Nov26;[Epubaheadofprint]PMID:17178443[PubMed-assuppliedbypublisher]5.KennethI.Aston,Guang-PengLi,BradyA.Hicks,BenjaminR.Sessions,BarryJ.Pate,DouglasS.Hammon,ThomasD.BunchandKennethL.White.2006.Thedevelopmentalcompetenceofbovinenucleartransferembryosderivedfromcowversusheifercytoplasts.AnimalReproductionScience.AnimalReprodScience.95(3-4):234-243.6.Guang-PengLi,ThomasD.Bunch,KennethL.White,LeeRickords,YingLiuandBenjaminR.Sessions.2006.Thedenudingandcentrifugationofmaturingbovineoocytesaltersoocytespindleintegrityandtheabilityofcytoplasmtosupportparthenogeneticandnucleartransferembryodevelopment.MoleReprodDev.73:446-451.7.AstonKI,LiGP,HicksBA,SessionsBR,PateBJ,HammonD,BunchTDandWhiteKL.2006.Effectofthetimeintervalbetweenfusionandactivationonnuclearstateanddevelopmentinvitroandinvivoofbovinesomaticnucleartransferembryos.Reproduction131:45-51.8.Guang-PengLi,YingLiu,ThomasD.Bunch,KennethL.White,KennethI.Aston.2005.AsymmetricdivisionofspindlemicrotubulesandmicrofilamentsduringbovinemeiosisfrommetaphaseItometaphaseIII.Mol.Reprod.Dev.71:220-226.9.G-PLi,YLiu,KLWhite,TDBunch.2005.Cytogeneticanalysisofdiploidyinclonedbovineembryosusinganimprovedairdrycaryotypingmethod.Theriogenology.2005Jun;63(9):2434-2444.10.WhiteKL,WoodsGL,VanderwallDK,LiGP,SessionsBR,BunchTD.2004.Whyclonehorsesandmules?Lessonslearnedfromthesuccessfulbirthsofclonedmulesfromahorse.IEEEEngineeringinmedicineandBiologyMagazine23(2):32-36.11.Guang-PengLi,ThomasD.Bunch,KennethL.White,KennethI.Aston,LoraN.Meerdo,BarryJ.Pate,BenjaminR.Sessions.2004.Development,chromosomalcompositionandallocationofinnercellmassandtrophectodermofbovinenucleartransferblastocystsaftercultureinconditionedmedia.MolReprodDev68:189-197.12.Guang-PengLi,KennethL.WhiteandThomasD.Bunch.2004.Reviewofenucleationmethodsandproceduresusedinanimalcloning:stateoftheart.CloningandStemCells6:5-14.13.G-PLi,WhiteKL,AstonKI,MeerdoLN,BunchTD.2004.Conditionedmediumincreasesthepolyploidcellcompositionofbovinesomaticcellnucleartransferredblastocysts.Reproduction127:221-228.14.DirkK.Vanderwall,GordonL.Woods,KennethI.Aston,ThomasD.Bunch,GuanpengLi,LoraN.MeerdoandKennethL.White.2004.Clonedhorsepregnanciesproducedusingadultcumuluscells.Reproduction,FertilityandDevelopment16(7)675–679.15.Guang-PengLi,G.E.Seidel,Jr.andE.L.Squires.2004.ImprovedcleavageofbovineICSIovaculturedinheparin-containingmedium.Theriogenology61(6):1077-1084.16.WoodsGL,WhiteKL,VanderwallDK,LiGP,AstonKI,BunchTD,MeerdoLN,PateBJ.2003.Amuleclonedfromfetalcellsbynucleartransfer.Science301(5636):1063.Epub2003May29.17.G-PLi,G.E.SeidelJr.,andE.L.Squires.2003.Intracytoplasmicsperminjectionofbovineoocyteswithstallionspermatozoa.Theriogenology,59:1143-1155.18.GuangpengLi,DayuanChen,LianLi,ZhimingHan,GESeidelJr.2002.Rabbitcloning:improvedfusionratesusingcytochalasinBinthefusionbuffer.MolReprodDev61(2):187-191.19.Guang-PengLi,Da-YuanChen,LiLian,Qing-YuanSun,Min-KangWang.Ji-SongLi,Zhi-MingHan.2001.Viablerabbitsderivedfromreconstructedoocytesbygerminalvesicletransferafterintracytoplasmicsperminjection(ICSI).MolReprodDev58:180-185.20.Guang-PengLi,Da-YuanChen,LiLian,Qing-YuanSun,Min-KangWang.2001.Mouse-rabbitgerminalvesicletransferrevealsthatfactorsregulatingoocytesmeioticprogressionarenotspecies-specificinmammals.JExpZool289:322-329.21.GuangpengLi,LianLi,MinkangWang,DayuanChen.2001.Maturationofthereconstitutedoocytesbygerminalvesicletransferinrabbitsandmice.Theriogenology56:855-866.22.WangMK,ChenDY,LiuJL,LiGP,SunQY.2001.InvitrofertilizationofmouseoocytesreconstructedbytransferofmetaphaseIIchromosomesresultsinlivebirths.Zygote9:9-14.23.WangMK,LiuJL,LiGP,LianL,ChenDY.2001.Sucrosepretreatmentforenucleation:Anefficientandnon-damagemethodforremovingthespindleofthemouseMIIoocyte.MolReprodDev58:432-436.24.Guang-PengLi,Jing-HeTan,Qing-YuanSun,Qing-GangMeng,Kui-ZhongYue,Xin-SenSun,Zi-YiLi,Hong-BinWang,Li-BinXu.2000.Clonedpigletsbornafternucleartransplantationofembryonicblastomeresintoporcineoocytesmaturedinvivo.Cloning,2(1):45-52.25.ChenDY,SunQY,LiuJL,LiGP,LianL,WangMK,HanZM,SongXF,LiJS,SunQ,ChenYC,ZhangYPandDingB.1999.Thegiantpanda(Ailuropodamelanoleuca)somaticnucleuscandedifferentiateinrabbitooplasmandsupportearlydevelopmentofthereconstructedegg.ScienceinChina(SeriesC),42:346-353.26.LiGuang-Peng,MengQG,WeiP,YuYS,ChangZL,TanJH.2001.Effectsofcycloheximide(CHX)oninvitromaturationofporcineoocytes.ActaZoologicaSinica47(6):684-690.27.LiGP,LianL,LianY,ChenDY.2001.Reproductionengineeringofdomesticcatandnondomesticfelidspecies.ZoologicalResearch22:413-420.28.LiGP,LanGC,LiuY,YuYS,SunXS,MaSF,TanJH.2000.Interspecificnucleartransferbetweenmouseandpig.ZoologicalResearch21:416-418.29.LiGP.LanGC,MaSF,TanJH.Thebehaviorofthecellmembraneofporcineoocytesmaturedinvitrointheelectricfield.ActaAnatomicaSinica,2000,21:145-148.30.LiGP,MengQG,WeiP,TanJH.Technicalmodificationsofinvitromaturationofporcineoocytes.ChineseJournalofVeterinaryScience,2000,12:32-35.31.LiGPCaiSX,XuLB,TanJH.2000.Studiesontheearlydevelopmentofmousetetraploidembryosproducedbyelectrofusion.Developmental&ReproductiveBiology,7(1):17-23.